According to a forthcoming publication in Nature , modern humans and Neanderthals interbred tens of thousands of years before than what had been previously reported. Using several different methods of DNA analysis and a greater amount of Neanderthal genomes, an international research team has reaffirmed the interbreeding between Neanderthals and modern humans that occurred previous to the 65.000 years thought so far: an estimated 100.000 years ago. Researchers suggest that some modern humans left Africa earlier than what we thought and mixed with Neanderthals. These modern humans probably became extinct and are therefore not among the ancestors of present-day humans.

Consistent with previous studies, modern humans appeared 200.000 years ago in Africa and spread through Eurasia around 65.000 years ago. Thanks to an unidirectional signal of Neanderthal DNA found in the genomes of humans outside Africa, it was also known that Neanderthals and modern humans had interbred by that time. The present study, however, brings to light the first evidence of the genetic contribution from modern humans to Neanderthals, a process that probably occurred in a previous migration.

The evidences of gene flow from modern humans into the Neanderthal genome are based on the analysis of one specific Neanderthal, whose remains were found in a cave in the Altai Mountains in southern Siberia, near the border between Russia and Mongolia. Two Neanderthals from European caves that were sequenced for this study — one from Croatia, another from Spain — both lack DNA derived from modern humans. The team also analyzed the genome of another extinct human, a Denisovan, whose remains (a finger and a tooth) were found in the same cave in the Altai Mountains as the Neanderthal bone. Unlike the Neanderthal individual, the Denisovan individual did not carry any modern human DNA. The researchers do not conclude that modern humans never mated with Denisovans or European Neanderthals, but that the signal they had found in the Altai Neanderthal probably comes from an interbreeding event that occurred after a linage with other modern humans from Europe a little more than 100,000 years ago.

Martin Kuhlwilm, first co-author of the article led by Sergi Castellano from the Max Planck Institute, identified the regions of the Altai Neanderthal genome that come from modern humans. “I was looking to see if I could find regions in the genome where the Neanderthal genome from Siberia has sequences resembling those in humans. We know that contemporary non-Africans have traces of Neanderthal in them, so they were not useful to us. So we instead used genomes of contemporary individuals from across Africa to identify mutations which most of them have in common. Some of these mutations occur together in regions of the Altai Neanderthal genome, a sign of interbreeding,” adds Kuhlwilm, who did this work at Max Planck Institute for Evolutionary Anthropology. Martin Kuhlwilm is currently conducting his postdoctoral research at the Institute of Evolutionary Biology (IBE), joint center of the Spanish National Research Council (CSIC) and the Pompeu Fabra University (UPF), in the Comparative Genomics Lab , a research group led by Tomàs Marquès-Bonet who, together with the group leader of the Paleogenomics Lab (IBE), Carles Lalueza-Fox, also took part in this study.

The modern human DNA sequences in the Altai Neanderthal appear to derive from a modern human group that separated early from other humans. The modern human who contributed genes to this particular Neanderthal individual must have come from a population that left Africa long before the migration of the ancestors of present-day Europeans and Asians from Africa less than 65,000 years ago, the scientists say. Thus, there must have been a long lag between when this group branched off the modern human family tree, roughly 200,000 years ago, and when they left their genetic mark in the Altai Neanderthal, about 100,000 years ago, before themselves being lost to extinction.

Reference Article: Kuhlwilm, M.; Gronau, I.; Hubisz, M.J.; de Filippo, C.; Prado, J.; Kircher, M.; Fu, Q.; Burbano, H.A.; Lalueza-Fox, C.; de la Rasilla, M.; Rosas, A.; Rudan, P.; Brajkovic, D.; Kucan, Z.; Gušic, I.; Marques-Bonet, T.; Andrés, A.M.; Viola, B.; Pääbo, S.; Meyer, M.; Siepel A.; and Castellano, S. (2016). Ancient gene flow from modern humans into Siberian Neanderthals. Nature